tau Protein (P301L, partial)

N° du produit ABIN6929400

Protéine Recombinant tau exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour tau Protein (P301L, partial) (ABIN6929400)

Antigène: tau (tau Protein)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Détail du produit

Attributs du protein: P301L, partial

Fonction: Active Human Recombinant Tau (K18), P301L mutant Protein Pre-formed Fibrils

Séquence: SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV LGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE

Specificité: ~15.1 kDa

Purification: Ion-exchange Purified

Biological Activity Comment: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: Lot specific

Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4

Stock: -80 °C

Détail du antigène

Antigène: tau (tau Protein)

Autre désignation: Tau

Sujet: Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10 % of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).

UniProt: P10636