CSNK1G1 Protein (His-GST)

N° du produit ABIN7317070

Protéine Recombinant CSNK1G1 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour CSNK1G1 Protein (His-GST) (ABIN7317070)

Antigène: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 94 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CSNK1G1 protéine est marqué à la His-GST.

Fonction: Recombinant Human CSNK1G1/CKI-gamma 1 Protein (His & GST Tag)

Séquence: Met 1-Lys 422

Attributs du produit: A DNA sequence encoding full length of human CSNK1G1 (NP_071331.2) (Met 1-Lys 422) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 4 nmol/min/mg using casein as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Autre désignation: CSNK1G1/CKI-gamma 1

Sujet: CK1gamma1,Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Thecasein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators ofsignal transductionpathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine-threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53 % -98 % identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates.

Poids moléculaire:

Calculated MW: 76.3 kDa

Observed MW: 66 kDa

ID gène: 53944

NCBI Accession: NP_071331

UniProt: Q9HCP0

Pathways: Signalisation Hedgehog