Cathepsin B Protein (CTSB) (AA 1-339) (His tag)

N° du produit ABIN7321072

Protéine Recombinant Cathepsin B exprimée dans HEK-293 Cells.

Aperçu rapide pour Cathepsin B Protein (CTSB) (AA 1-339) (His tag) (ABIN7321072)

Antigène: Cathepsin B (CTSB)

Type de proteíne: Recombinant

Origine: Rat

Source: HEK-293 Cells

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 1-339

Purification/Conjugué: Cette Cathepsin B protéine est marqué à la His tag.

Fonction: Recombinant Rat Cathepsin B/CTSB Protein (His Tag)

Séquence: Met1-Phe339

Attributs du produit: A DNA sequence encoding the rat Ctsb (NP_072119.2) (Met1-Phe339) was expressed with a polyhistidine tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 25 mM Tris, 150 mM NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Cathepsin B (CTSB)

Autre désignation: Cathepsin B/CTSB

Sujet: CTSB,Cathepsin B,Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

Poids moléculaire: 37.1 kDa

NCBI Accession: NP_072119

UniProt: F7FAT8

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades