Cathepsin Z Protein (CTSZ) (His tag)

N° du produit ABIN7194694

Cette protéine Recombinant Cathepsin Z est exprimée dans HEK-293 Cells.

Aperçu rapide pour Cathepsin Z Protein (CTSZ) (His tag) (ABIN7194694)

Antigène: Cathepsin Z (CTSZ)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Pureté: > 95 % as determined by SDS-PAGE

Détail du produit

Purification/Conjugué: Cette Cathepsin Z protéine est marqué à la His tag.

Fonction: Recombinant Mouse Cathepsin Z Protein (His Tag)(Active)

Séquence: Met 1-Val 306

Attributs du produit: A DNA sequence encoding the mouse CTSZ (NP_071720.1) precursor (Met 1-Val 306) was expressed with a polyhistidine tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (R&D Systems, Catalog # ES005). The specific activity is >1,200 pmoles/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Cathepsin Z (CTSZ)

Autre désignation: Cathepsin Z

Sujet:

Background: Cathepsin Z (CTSZ), also known as Cathepsin X or CATX, belongs to the C1 family of lysosomal cysteine proteases. Its gene structure and activity properties show several unique features that distinguish it clearly from other human cysteine proteases. It has a very short pro-region that shows no similarity to those of other cathepsins and a three-residue insertion motif that forms a characteristic ‘mini loop’. Cathepsin Z exhibits mono- and di-peptidase activity at its C-terminus, and in contrast to cathepsin B, it does not act as an endopeptidase. It is restricted to the cells of theimmune system, predominantly monocytes, macrophages and dendritic cells. Cathepsin Z is widely expressed in human tissues, suggesting that this enzyme could be involved in the normal intracellular protein degradation taking place in all cell types. It is capable to cleave regulatory motifs at C-terminus affecting the function of targeted molecules. Cathepsin X may regulate also the maturation of dendritic cells, a process, which is crucial in the initiation of adaptive immunity. Furthermore, higher levels of Cathepsin Z are also found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa, especially after infection by Helicobacter pylori. Cathepsin Z is also ubiquitously distributed in cancer cell lines and in primary tumors from different sources, suggesting that this enzyme may participate in tumor progression.

Synonym: AI787083;AU019819;CTSX;D2Wsu143e

Poids moléculaire: 33.2 kDa

NCBI Accession: NP_071720

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones