CD40 Protein (CD40) (His tag)

N° du produit ABIN7319657

Protéine Recombinant CD40 exprimée dans Human Cells.

Aperçu rapide pour CD40 Protein (CD40) (His tag) (ABIN7319657)

Antigène: CD40

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Human Cells

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette CD40 protéine est marqué à la His tag.

Fonction: Recombinant Human CD40/TNFRSF5 Protein (His Tag)(Active)

Séquence: Glu21-Arg193

Attributs du produit: Recombinant Human CD40 is produced by our Mammalian expression system and the target gene encoding Glu21-Arg193 is expressed with a 6His tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human CD40L(Cat: PKSH033725) at 5μg/ml(100 μl/well) can bind Human CD40-His. The ED50 of Human CD40-His is 44.004 ug/ml .

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CD40

Autre désignation: CD40/TNFRSF5

Sujet:

Background: CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand (CD40L) leads to aggregation of CD40 Molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins (adaptor proteins) have been identified to serves as mediators of the signal transduction. CD40 plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Synonym: Tumor Necrosis Factor Receptor Superfamily member 5, B-Cell Surface Antigen CD40, Bp50, CD40L Receptor, CDw40, CD40, TNFRSF5

Poids moléculaire: 20.2 kDa

UniProt: P25942

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints