CSF1R Protéine

N° du produit ABIN7318000

Cette protéine Recombinant CSF1R est exprimée dans HEK-293 Cells.

Aperçu rapide pour CSF1R Protéine (ABIN7318000)

Antigène: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: ELISA, Cell Culture (CC)

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Fonction: Recombinant Human CSF1R/CD115 Protein

Séquence: Met 1-Glu 512

Attributs du produit: The mature form of human M-CSFR (NP_005202.2) extracellular domain (Met 1-Glu 512) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Immobilized sMCSFR at 10 μg/ml (100 μl/well) can bind biotinylated human CSF1-His, The EC₅₀ of biotinylated human CSF1-His is 32-74. 8 ng/ml. 2. Measured by its ability to inhibit the human CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is typically 0.3-1. 5 μg/mL in the presence of 3 ng/mL of rhM-CSF.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Autre désignation: CSF1R/CD115

Sujet: C-FMS;CD115;CSF-1 receptor;CSF-1-R;CSF-1R;CSF1R;FIM2;FMS;HDLS;M-CSF-R;MCSF Receptor ;Macrophage colony-stimulating factor 1 receptor;Proto-oncogene c-Fms,M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation.

Poids moléculaire:

Calculated MW: 55.1 kDa

Observed MW: 80-90 kDa

ID gène: 1436

NCBI Accession: NP_005202

UniProt: P07333

Pathways: Signalisation RTK, Inositol Metabolic Process, Cell-Cell Junction Organization