C5A Protein (AA 678-751)

N° du produit ABIN7319677

Cette protéine Recombinant C5A est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour C5A Protein (AA 678-751) (ABIN7319677)

Antigène: C5A (C5a) (Complement Component 5a (C5a))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 678-751

Fonction: Recombinant Human Complement C5a Protein

Séquence: Thr678-Arg751

Attributs du produit: Recombinant Human C5a is produced by our E.coli expression system and the target gene encoding Thr678-Arg751 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: C5A (C5a) (Complement Component 5a (C5a))

Autre désignation: Complement C5a

Sujet: C5Da,C5a,C5a anaphylatoxin,CPAMD4,Complement C5,ECLZB,Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 α-chain. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60 % and 54 % aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

Poids moléculaire:

Calculated MW: 8.4 kDa

Observed MW: 12 kDa

ID gène: 727

UniProt: P01031

Pathways: Système du Complément, Carbohydrate Homeostasis