EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST)

N° du produit ABIN7317076

Cette protéine Recombinant EPH Receptor A4 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour EPH Receptor A4 Protein (EPHA4) (AA 570-986) (His-GST) (ABIN7317076)

Antigène: EPH Receptor A4 (EPHA4)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: ELISA

Pureté: > 99 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 570-986

Purification/Conjugué: Cette EPH Receptor A4 protéine est marqué à la His-GST.

Fonction: Recombinant Human EphA4 Protein (aa 570-986, His &GST Tag)

Séquence: Ser 570-Val 986

Attributs du produit: A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized human EPHA4 (aa 570-986)at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10 % glycerol, 3 mM DTT

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: EPH Receptor A4 (EPHA4)

Autre désignation: EphA4

Sujet: EK8;EPH-like kinase 8;EPHA4;Ephrin type-A receptor 4;HEK8;SEK;TYRO1;Tyrosine-protein kinase TYRO1;Tyrosine-protein kinase receptor SEK;hEK8,EPH receptor A4 (ephrin type-A receptor 4); also known as EphA4; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus; and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling; which is triggered by ephrins7; probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands; which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

Poids moléculaire:

Calculated MW: 75.0 kDa

Observed MW: 67 kDa

ID gène: 2043

UniProt: P54764

Pathways: Signalisation RTK