GZMB Protein (His tag)

N° du produit ABIN7195987

Cette protéine Recombinant GZMB est exprimée dans HEK-293 Cells.

Aperçu rapide pour GZMB Protein (His tag) (ABIN7195987)

Antigène: GZMB (Granzyme B (GZMB))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 97 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette GZMB protéine est marqué à la His tag.

Fonction: Recombinant Human Granzyme B/GZMB Protein (His Tag)

Séquence: Met 1-Tyr 247

Attributs du produit: A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: GZMB (Granzyme B (GZMB))

Autre désignation: Granzyme B/GZMB

Sujet: CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT,Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

Poids moléculaire:

Calculated MW: 27.0 kDa

Observed MW: 36 kDa

NCBI Accession: NP_004122

Pathways: Apoptose, Caspase Cascade in Apoptosis