Hemopexin Protein (HPX) (His tag)

N° du produit ABIN7317735

Protéine Recombinant Hemopexin exprimée dans HEK-293 Cells.

Aperçu rapide pour Hemopexin Protein (HPX) (His tag) (ABIN7317735)

Antigène: Hemopexin (HPX)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: ELISA

Pureté: > 96 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Hemopexin protéine est marqué à la His tag.

Fonction: Recombinant Human Hemopexin/HPX Protein (His Tag)

Séquence: Met 1-His 462

Attributs du produit: A DNA sequence encoding the human HPX (NP_000604.1) (Met 1-His 462) with a C-terminal polyhistidine tag was expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant human Hemopexin binds > 10 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Hemopexin (HPX)

Autre désignation: Hemopexin/HPX

Sujet: Hemopexin,Hpx,Hpxn,Hemopexin (HPX) is plasma glycoprotein belongs to the family of the acute-phase proteins whose synthesis is induced after an inflammatory event. Hemopexin with two four-bladed beta -propeller folds has been found in other proteins including collagenases and provides sites for protein-protein interactions. The liver is the major synthesizing organ. Hemopexin participates in maintaining and recycling the iron pool by utilizing its high binding affinity toward heme composed of protoporphyrin IX and iron. It also functions in preventing oxidation caused by heme after hemolysis. Hydrophobic heme molecules can intercalate into lipid membranes and participate in the oxidation of lipid membrane components through the Fenton reaction resulting in lipid peroxidation. Hemopexin undergoes a conformational change upon the binding of heme. The conformational change allows hemopexin to interact with a specific receptor, forming a complex which is then internalized. Heme concentrations in plasma increase after hemolysis, which is associated with several pathological conditions such as reperfusion injury and ischemia.

Poids moléculaire:

Calculated MW: 50.7 kDa

Observed MW: 70-75 kDa

ID gène: 3263

NCBI Accession: NP_000604

UniProt: P02790

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response