Hemopexin Protein (HPX) (His tag)

N° du produit ABIN7320339

Cette protéine Recombinant Hemopexin est exprimée dans HEK-293 Cells.

Aperçu rapide pour Hemopexin Protein (HPX) (His tag) (ABIN7320339)

Antigène: Hemopexin (HPX)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Pureté: > 98 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Hemopexin protéine est marqué à la His tag.

Fonction: Recombinant Mouse Hemopexin/HPX Protein (His Tag)

Séquence: Met 1-Gln 460

Attributs du produit: A DNA sequence encoding the full length of mouse HPX (NP_059067.2) (Met 1-Gln 460) was expressed, with a polyhistidine tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant mouse Hemopexin binds > 15 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of mouse Hemopexin.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM MES, 150 mM NaCl, pH 6.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Hemopexin (HPX)

Autre désignation: Hemopexin/HPX

Sujet: Hemopexin,Hpx,Hpxn,Hemopexin (HPX) is plasma glycoprotein belongs to the family of the acute-phase proteins whose synthesis is induced after an inflammatory event. Hemopexin with two four-bladed beta -propeller folds has been found in other proteins including collagenases and provides sites for protein-protein interactions. The liver is the major synthesizing organ. Hemopexin participates in maintaining and recycling the iron pool by utilizing its high binding affinity toward heme composed of protoporphyrin IX and iron. It also functions in preventing oxidation caused by heme after hemolysis. Hydrophobic heme molecules can intercalate into lipid membranes and participate in the oxidation of lipid membrane components through the Fenton reaction resulting in lipid peroxidation. Hemopexin undergoes a conformational change upon the binding of heme. The conformational change allows hemopexin to interact with a specific receptor, forming a complex which is then internalized. Heme concentrations in plasma increase after hemolysis, which is associated with several pathological conditions such as reperfusion injury and ischemia.

Poids moléculaire:

Calculated MW: 50.4 kDa

Observed MW: 60-70 kDa

NCBI Accession: NP_059067

UniProt: Q91X72

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response