IL1A Protein (AA 115-270) (His tag)

N° du produit ABIN7320583

Cette protéine Recombinant IL1A est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL1A Protein (AA 115-270) (His tag) (ABIN7320583)

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 115-270

Purification/Conjugué: Cette IL1A protéine est marqué à la His tag.

Fonction: Recombinant Mouse IL-1A/IL-1α Protein

Séquence: Ser115-Ser270

Attributs du produit: Recombinant Mouse Interleukin-1 alpha is produced by our E.coli expression system and the target gene encoding Ser115-Ser270 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce D10.G4.1 cells proliferation.The ED₅₀ for this effect is <5 pg/mL.The specific activity of recombinant mouse IL-1 alpha is > 2 x 10⁸ IU/mg.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: IL-1A/IL-1alpha

Sujet: Hematopoietin,IL1F,Hematopoietin-1,IL1,IL1-A,IL1F1,IL-1α,Preinterleukin 1 Alpha,Pro-Interleukin-1-Alpha,Il-1a,Interleukin-1 alpha,IL-1 alpha,Il1a,Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25 % homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

Poids moléculaire:

Calculated MW: 18.9 kDa

Observed MW: 20 kDa

UniProt: P01582

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints