IL-18 Protein (AA 36-192) (His tag)

N° du produit ABIN7320581

Protéine Recombinant IL-18 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-18 Protein (AA 36-192) (His tag) (ABIN7320581)

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 98 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 36-192

Purification/Conjugué: Cette IL-18 protéine est marqué à la His tag.

Fonction: Recombinant Mouse Interleukin-18/IL-18 Protein (His Tag)

Séquence: Asn36-Ser192

Attributs du produit: Recombinant Mouse Interleukin-18 is produced by our E.coli expression system and the target gene encoding Asn36-Ser192 is expressed with a 6His tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce IFN gamma secretion in KG-1 cells.The ED₅₀ for this effect is <0.5 μg/mL.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Autre désignation: Interleukin-18/IL-18

Sujet: Interleukin,IL-1F,Ig,Il-Igif,Il-18,IFN-gamma-inducing factor,Il,IL-1 gamma,IL-1F4,IL-1g,Interferon gamma-inducing factor,Interferon-gamma-inducing factor,Interleukin-1 gamma,Interleukin-18,Igif,Il18,Igif,Il-,Il-18,Interleukin-18 (IL-18)is a protein which belongs to the IL-1 family. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature mouse IL-18 shares 63 % and 91 % amino acid sequence identity with mouse and rat IL-18, respectively. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils.

Poids moléculaire:

Calculated MW: 19.1 kDa

Observed MW: 18 kDa

ID gène: 16173

UniProt: P70380

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome