IL2 Receptor beta Protéine

N° du produit ABIN7196471

Protéine Recombinant IL2 Receptor beta exprimée dans HEK-293 Cells.

Aperçu rapide pour IL2 Receptor beta Protéine (ABIN7196471)

Antigène: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Fonction: Recombinant Human CD122/IL-2RB Protein (Active)

Séquence: Met 1-Asp 239

Attributs du produit: The mature form of human IL2 Rβ (NP_000869.1) extracellular domain (Met 1-Asp 239) with five amino acids (DDDDK) at the C-terminus was expressed and purified.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA. Immobilized human IL2Rb at 10 μg/ml (100 μl/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 μg/mL.2. Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1-4μg/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 100 mM NaCl, 50 mM Tris, pH 7.5

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Autre désignation: CD122/IL-2RB

Sujet:

Background: Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-, prepro B cells, fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

Synonym: CD122,IL15RB,P70-75

Poids moléculaire: 25.2 kDa

NCBI Accession: NP_000869

Pathways: Signalistation JAK/STAT, Growth Factor Binding