IL-31 Protein (AA 24-164) (His tag)

N° du produit ABIN7318671

Cette protéine Recombinant IL-31 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IL-31 Protein (AA 24-164) (His tag) (ABIN7318671)

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 98 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 24-164

Purification/Conjugué: Cette IL-31 protéine est marqué à la His tag.

Fonction: Recombinant Human Interleukin-31/IL-31 Protein

Séquence: Ser24-Thr164

Attributs du produit: Recombinant Human Interleukin-31 is produced by our E.coli expression system and the target gene encoding Ser24-Thr164 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to induce A549 cells proliferation. The ED₅₀ for this effect is < 40 ng/mL

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Autre désignation: Interleukin-31/IL-31

Sujet: Interleukin,IL31,IL-31,Interleukin-31,IL 31,Interleukin 31,Human Interleukin 31 (IL-31) is a cytokine containing a four-helix bundle structure. It shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. Human IL-31 cDNA encodes a 164 amino acid precursor that contains a 23 amino acid signal peptide and a 141 amino acid mature protein. Human and mouse IL-31 share 24 % sequence identity in the mature region. IL-31 is mainly associated with activated T cells and is preferentially expressed by type 2 helper T cells (Th2). IL-31 signals via a heterodimeric receptor complex composed of a gp130 related molecule termed IL-31RA (also GPL and GLMR) and an Oncostatin M receptor (OSM Rβ). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFNγ on monocytes. GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Studies have shown that IL31 induces severe pruritis (itching) and dermatitis in transgenic mice.

Poids moléculaire:

Calculated MW: 16.8 kDa

Observed MW: 18 kDa

ID gène: 386653

UniProt: Q6EBC2