IL-4 Protein (AA 21-140) (His tag)

N° du produit ABIN7320597

Protéine Recombinant IL-4 exprimée dans HEK-293 Cells.

Aperçu rapide pour IL-4 Protein (AA 21-140) (His tag) (ABIN7320597)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Application: Cell Culture (CC)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 21-140

Purification/Conjugué: Cette IL-4 protéine est marqué à la His tag.

Fonction: Recombinant Mouse Interleukin-4/IL-4 Protein (His Tag)

Séquence: His21-Ser140

Attributs du produit: Recombinant Mouse Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His21-Ser140 is expressed with a 6His tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using M-NFS-60 mouse lymphoblast cells. The ED₅₀ for this effect is 104 pg/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: Interleukin-4/IL-4

Sujet: Interleukin,B-cell growth factor,B-cell stimulatory factor,Lymphocyte stimulatory factor,BCGF1,BSF-1,B-cell growth factor 1,B-cell IgG differentiation factor,B-cell stimulatory factor 1,BCGF-1,BSF1,IGG1 induction factor,Interleukin-4,Lymphocyte stimulatory factor 1,Il-4,Il4,Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

Poids moléculaire:

Calculated MW: 14.6 kDa

Observed MW: 15-19 kDa

ID gène: 16189

UniProt: P07750

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation