c-MET Protein (AA 956-1390) (His-GST)

N° du produit ABIN7317093

Cette protéine Recombinant c-MET est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour c-MET Protein (AA 956-1390) (His-GST) (ABIN7317093)

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: ELISA

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 956-1390

Purification/Conjugué: Cette c-MET protéine est marqué à la His-GST.

Fonction: Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)

Séquence: Lys956-Ser1390

Attributs du produit: A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. 2. Immobilized human HGFR (aa 956-1390) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 3 mM DTT

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Autre désignation: c-MET/HGFR

Sujet: AUTS9,DFNB97,HGFR,RCCP2,c-Met,Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Poids moléculaire:

Calculated MW: 76.8 kDa

Observed MW: 68 kDa

ID gène: 4233

UniProt: P08581

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor