Nemo-Like Kinase Protein (NLK) (His-GST)

N° du produit ABIN7317069

Cette protéine Recombinant Nemo-Like Kinase est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour Nemo-Like Kinase Protein (NLK) (His-GST) (ABIN7317069)

Antigène: Nemo-Like Kinase (NLK)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 91 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Nemo-Like Kinase protéine est marqué à la His-GST.

Fonction: Recombinant Human NLK Protein (His & GST Tag)

Séquence: Val121-Glu 527

Attributs du produit: A DNA sequence encoding the human NLK (Q9UBE8) (Val121-Glu527) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: Nemo-Like Kinase (NLK)

Autre désignation: NLK

Sujet: LAK1,NLK,Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.

Poids moléculaire:

Calculated MW: 74.1 kDa

Observed MW: 73 kDa

ID gène: 51701

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway