NOG Protein (AA 28-232) (His tag)

N° du produit ABIN7320797

Cette protéine Recombinant NOG est produite dans HEK-293 Cells.

Aperçu rapide pour NOG Protein (AA 28-232) (His tag) (ABIN7320797)

Antigène: NOG (Noggin (NOG))

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Application: Cell Culture (CC)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 28-232

Purification/Conjugué: Cette NOG protéine est marqué à la His tag.

Fonction: Recombinant Mouse Noggin/NOG Protein (His Tag)

Séquence: Gln28-Cys232

Attributs du produit: Recombinant Mouse Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed with a 6His tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, 5 mM EDTA, 5 % Trehalose, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: NOG (Noggin (NOG))

Autre désignation: Noggin/NOG

Sujet: Nog,Noggin,Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.

Poids moléculaire:

Calculated MW: 23.9 kDa

Observed MW: 30 kDa

UniProt: P97466

Pathways: Stem Cell Maintenance, Tube Formation