NOG Protein (AA 28-232)

N° du produit ABIN7319511

Cette protéine Recombinant NOG est exprimée dans HEK-293 Cells.

Aperçu rapide pour NOG Protein (AA 28-232) (ABIN7319511)

Antigène: NOG (Noggin (NOG))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: Cell Culture (CC)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 28-232

Fonction: Recombinant Human Noggin/NOG Protein

Séquence: Gln28-Cys232

Attributs du produit: Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 10-100ng/mL in the presence of 200 ng/mL of Recombinant Human BMP-4(#CR93).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: NOG (Noggin (NOG))

Autre désignation: Noggin/NOG

Sujet: NOG,Noggin,Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99 % , 99 %, 98 %, 97 % and 89 % aa sequence identity with mouse, rat bovine, equine and chicken Noggin, respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.

Poids moléculaire:

Calculated MW: 23 kDa

Observed MW: 25-33 kDa

ID gène: 9241

UniProt: Q13253

Pathways: Stem Cell Maintenance, Tube Formation