MGMT Protein (His tag)

N° du produit ABIN7318122

Cette protéine Recombinant MGMT est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MGMT Protein (His tag) (ABIN7318122)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette MGMT protéine est marqué à la His tag.

Fonction: Recombinant Human MGMT Protein (His Tag)

Séquence: Met 1-Asn207

Attributs du produit: Recombinant Human Methylated-DNA--protein-cysteine methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl,1 mM DTT,1 mM EDTA,500 mM NaCl,0.1 % Trition X-100, pH 8.0.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet: 6-O-methylguanine-DNAmethyltransferase,MGMT,Methylated-DNA--protein-cysteine methyltransferase,O-6-methylguanine-DNA-alkyltransferase,MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Poids moléculaire:

Calculated MW: 23.8 kDa

Observed MW: 25 kDa

ID gène: 4255

UniProt: P16455

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus