Podoplanin Protein (PDPN) (His-Fc tag)

N° du produit ABIN7320346

Cette protéine Recombinant Podoplanin est exprimée dans HEK-293 Cells.

Aperçu rapide pour Podoplanin Protein (PDPN) (His-Fc tag) (ABIN7320346)

Antigène: Podoplanin (PDPN)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Application: ELISA

Pureté: > 97 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Podoplanin protéine est marqué à la His-Fc tag.

Fonction: Recombinant Mouse Podoplanin/PDPN Protein (His & Fc Tag)

Séquence: Met 1-Leu 141

Attributs du produit: A DNA sequence encoding the extracellular domain (Met 1-Leu 141) of mouse PDPN (NP_034459.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized mouse PDPN-Fch at 10 μg/ml (100 μl/well) can bind biotinylated human CLEC1B-His, The EC₅₀ of biotinylated human CLEC1B-His is 0.04-0.08 μg/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin/PDPN

Sujet: Aggrus,Glycoprotein 38,Gp38,OTS-8,PA2.26 antigen,Podoplanin,RANDAM-2,T1-alpha,T1a,T1alpha,Podoplanin, also known asPDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.

Poids moléculaire:

Calculated MW: 40.6 kDa

Observed MW: 60-65 kDa

ID gène: 14726

NCBI Accession: NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport