PTPN12 Protein (AA 1-355) (His-GST)

N° du produit ABIN7317538

Cette protéine Recombinant PTPN12 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour PTPN12 Protein (AA 1-355) (His-GST) (ABIN7317538)

Antigène: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 1-355

Purification/Conjugué: Cette PTPN12 protéine est marqué à la His-GST.

Fonction: Recombinant Human PTPN12 Protein (aa 1-355, His & GST Tag)

Séquence: Met 1-Gln355

Attributs du produit: A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate, R&D Systems, Catalog # ES006. The specific activity is > 15 μmoles/min/mg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Autre désignation: PTPN12

Sujet: PTP-PEST,PTPG1,PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.

Poids moléculaire:

Calculated MW: 69.4 kDa

Observed MW: 64 kDa

ID gène: 5782

UniProt: Q05209