Ret Proto-Oncogene Protein (RET) (AA 658-1114) (His-GST)

N° du produit ABIN7317056

Cette protéine Recombinant Ret Proto-Oncogene est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour Ret Proto-Oncogene Protein (RET) (AA 658-1114) (His-GST) (ABIN7317056)

Antigène: Ret Proto-Oncogene (RET)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 658-1114

Purification/Conjugué: Cette Ret Proto-Oncogene protéine est marqué à la His-GST.

Fonction: Recombinant Human RET Kinase Protein (aa 658-1114, His & GST Tag)

Séquence: His 658-Ser 1114

Attributs du produit: A DNA sequence encoding the cytoplasmic domain of human RET (P07949-1) (His 658-Ser 1114) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 17 nmol/min/mg using synthetic TRK-C-derived Peptide (R11-VYSTDYYRLFNPS) as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: Ret Proto-Oncogene (RET)

Autre désignation: RET Kinase

Sujet: CDHF12,CDHR16,HSCR1,MEN2A,MEN2B,MTC1,PTC,RET-ELE1,RET51,RET proto-oncogene, also known as RET, is a cell-surface molecule that transduce signals for cell growth and differentiation. It contains 1 cadherin domain and 1 protein kinase domain. RET proto-oncogene belongs to the protein kinase superfamily, tyr protein kinase family. RET proto-oncogene is involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. It phosphorylates PTK2/FAK1 and regulates both cell death/survival balance and positional information. RET is required for the molecular mechanisms orchestration during intestine organogenesis, involved in the development of enteric nervous system and renal organogenesis during embryonic life, promotes the formation of Peyer's patch-like structures, modulates cell adhesion via its cleavage, involved in the development of the neural crest. RET proto-oncogene is active in the absence of ligand, triggering apoptosis. RET acts as a dependence receptor, in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. It also regulates nociceptor survival and size, triggers the differentiation of rapidly adapting (RA) mechanoreceptors, mediated several diseases such as neuroendocrine cancers.

Poids moléculaire:

Calculated MW: 76.7 kDa

Observed MW: 70 kDa

ID gène: 5979

UniProt: P07949

Pathways: Signalisation RTK, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation