TEK Protein (AA 771-1124) (His-GST)

N° du produit ABIN7317790

Cette protéine Recombinant TEK est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour TEK Protein (AA 771-1124) (His-GST) (ABIN7317790)

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: ELISA

Pureté: > 92 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 771-1124

Purification/Conjugué: Cette TEK protéine est marqué à la His-GST.

Fonction: Recombinant Human Tie2/CD202b Protein (His & GST Tag)

Séquence: Gln771-Ala1124

Attributs du produit: A DNA sequence encoding the human TEK (NP_000450) (Gln771-Ala1124) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. No Kinase Activity. 2. Immobilized human TEK (aa 771-1124) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.31-20 μg/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Autre désignation: Tie2/CD202b

Sujet: CD202B,TIE-2,TIE2,VMCM,VMCM1,TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Poids moléculaire:

Calculated MW: 68.3 kDa

Observed MW: 64 kDa

ID gène: 7010

NCBI Accession: NP_000450

UniProt: Q02763

Pathways: Signalisation RTK, Growth Factor Binding