UBE2I Protéine

N° du produit ABIN7198588

Cette protéine Recombinant UBE2I est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour UBE2I Protéine (ABIN7198588)

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Fonction: Recombinant Human UBE2I/UBC9 Protein

Séquence: Met 1-Ser 158

Attributs du produit: A DNA sequence encoding the human UBE2I (P63279) (Met 1-Ser 158) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Stérilité: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Autre désignation: UBE2I/UBC9

Sujet: SUMO-Conjugating Enzyme UBC9,SUMO-Protein Ligase,UBC9,UBCE9,UBE2I,Ubiquitin Carrier Protein 9,Ubiquitin Carrier Protein I,Ubiquitin-Conjugating Enzyme E2 I,Ubiquitin-Protein Ligase I,p18,UBE2I is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.

Poids moléculaire:

Calculated MW: 18.0 kDa

Observed MW: 18 kDa

ID gène: 7329

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway