Sonic Hedgehog Protein (SHH) (AA 25-198)

N° du produit ABIN7320696

Protéine Recombinant Sonic Hedgehog exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Sonic Hedgehog Protein (SHH) (AA 25-198) (ABIN7320696)

Antigène: Sonic Hedgehog (SHH)

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 25-198

Fonction: Recombinant Mouse Sonic Hedgehog/SHH Protein

Séquence: Cys25-Gly198

Attributs du produit: Recombinant Mouse Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys25-Gly198 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Sonic Hedgehog (SHH)

Autre désignation: SHH

Sujet: 9530036O11Rik,Dsh,HHG-1,Hhg1,Hx,Hxl3,M10008,SHH,Sonic Hedgehog Protein,Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. Mouse Shh-N is 99 % , 98 %, and 100 % aa identical to human, rat and gerbil Shh-N, respectively.

Poids moléculaire:

Calculated MW: 19.8 kDa

Observed MW: 20 kDa

ID gène: 20423

UniProt: Q62226

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development