Sonic Hedgehog Protein (SHH)

N° du produit ABIN7320696

Protéine Recombinant Sonic Hedgehog exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Sonic Hedgehog Protein (SHH) (ABIN7320696)

Antigène: Sonic Hedgehog (SHH)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by SDS-PAGE

Détail du produit

Fonction: Recombinant Mouse Sonic Hedgehog/SHH Protein (Active)

Séquence: Cys25-Gly198

Attributs du produit: Recombinant Mouse Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys25-Gly198 is expressed.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Mouse SHH at 1μg/ml(100 μl/well) can bind Human BOC-His(Cat: PKSH032123). The ED50 of Mouse SHH is 29 ug/ml .

Information d'application

Commentaires:

20 kDa

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Sonic Hedgehog (SHH)

Autre désignation: SHH

Sujet:

Background: Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. Mouse Shh-N is 99 % , 98 %, and 100 % aa identical to human, rat and gerbil Shh-N, respectively.

Synonym: Sonic Hedgehog Protein, SHH, HHG-1, SHH,9530036O11Rik, Dsh, Hhg1, Hx, Hxl3, M10008

Poids moléculaire: 19.8 kDa

UniProt: Q62226

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development