Sonic Hedgehog Protein (SHH)

N° du produit ABIN7319453

Protéine Recombinant Sonic Hedgehog exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Sonic Hedgehog Protein (SHH) (ABIN7319453)

Antigène: Sonic Hedgehog (SHH)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Fonction: Recombinant Human Sonic Hedgehog/SHH Protein (C24II)(Active)

Séquence: Cys24-Gly197(Cys24Ile-Ile)

Attributs du produit: Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197(Cys24Ile-Ile) is expressed.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human SHH at 1μg/ml(100 μl/well) can bind Human BOC-His(Cat: PKSH032123).

Information d'application

Commentaires:

20 kDa

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 1 mM DTT, pH 7.5.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Sonic Hedgehog (SHH)

Autre désignation: SHH

Sujet:

Background: Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45 kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the ""all signaling"" capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.

Synonym: Sonic Hedgehog Protein, SHH, HHG-1, SHH

Poids moléculaire: 19.8 kDa

UniProt: Q15465

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development