Annexin V Protein (AA 2-320)

N° du produit ABIN7273831

Cette protéine Recombinant Annexin V est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Annexin V Protein (AA 2-320) (ABIN7273831)

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 2-320

Fonction: Human Annexin V/ANXA5 Protein

Séquence: Ala2-Asp320

Attributs du produit: Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag.It contains Ala2-Asp320.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Annexin V on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Annexin V is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 240 mM NaCl ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Autre désignation: Annexin V

Sujet: Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.

Poids moléculaire: 35.9 kDa same as Bis-Tris PAGE result.

UniProt: P08758

Pathways: Apoptose