AXL Protein (AA 33-442) (His tag)

N° du produit ABIN7273881

Cette protéine Recombinant AXL est produite dans HEK-293 Cells.

Aperçu rapide pour AXL Protein (AA 33-442) (His tag) (ABIN7273881)

Antigène: AXL (AXL Receptor tyrosine Kinase (AXL))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 33-442

Purification/Conjugué: Cette AXL protéine est marqué à la His tag.

Fonction: Human AXL Protein

Séquence: Glu33-Ser442

Attributs du produit: Recombinant Human AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Ser442.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human AXL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.,Human AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-Axl Ab., hFc Tag on ProA-Biosensor can bind Human Axl, His Tag with an affinity constant of 0.20 μM as determined in BLI assay (Gator® Prime).,The purity of Human AXL is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: AXL (AXL Receptor tyrosine Kinase (AXL))

Autre désignation: AXL

Sujet: Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Poids moléculaire: 45.4 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.

UniProt: P30530

Pathways: Signalisation RTK, Cellular Response to Molecule of Bacterial Origin