BMP2 Protein (AA 283-396)

N° du produit ABIN7273975

Cette protéine Recombinant BMP2 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour BMP2 Protein (AA 283-396) (ABIN7273975)

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain, Chien, Rat, Souris, Macaque rhésus

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE

Détail du produit

Attributs du protein: AA 283-396

Fonction: Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

Séquence: Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine)

Attributs du produit: Recombinant Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein is expressed from E.coli without tag.It contains Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine).

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.5 EU per μg by the LAL method.

Biological Activity Comment: Human/Rhesus macaque/Mouse/Rat/Canine BMP2 on Bis-Tris PAGE under Non reduced (N) condition and reduced condition. The purity is greater than 95%.,Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 40-200 ng/mL (QC Test).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 100 mM Acetic Acid ( pH 2.8). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 100 mM Acetic Acid ( pH 2.8). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Autre désignation: BMP2

Sujet: Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-β (TGF-β) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs).

Poids moléculaire: 12.90 kDa same as Bis-Tris PAGE result.

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation