M-CSF/CSF1 Protein (AA 33-190)

N° du produit ABIN7274423

Protéine Recombinant M-CSF/CSF1 exprimée dans HEK-293 Cells.

Aperçu rapide pour M-CSF/CSF1 Protein (AA 33-190) (ABIN7274423)

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 33-190

Fonction: Human M-CSF/CSF-1 Protein

Séquence: Glu33-Asn190

Attributs du produit: Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 without tag.It contains Glu33-Asn190.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).,Immobilized Human M-CSF, No Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 13.5ng/ml determined by ELISA.,Human M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using RAW264.7 cells.The ED50-for this effect is 1-10 ng/mL.,The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Autre désignation: M-CSF

Sujet: CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

Poids moléculaire: 18.40 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.

UniProt: P09603

Pathways: Signalisation RTK