CNTF Protéine

N° du produit ABIN7491826

Protéine Recombinant CNTF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CNTF Protéine (ABIN7491826)

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Cell Culture (CC)

Pureté: ≥98 % (HPLC)

Détail du produit

Fonction: Recombinant rat CNTF protein

Séquence: AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Specificité: Toxin target: Leukemia Inhibitory Factor (LIF) receptor, gp130, CNTFR

niveau d'endotoxine: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: CNTF is a potent neurotrophic factor that was originally characterized as a survival factor for chick ciliary neurons. CNTF supports growth and survival of DRG, motor and sympathetic neurons1-4.

Information d'application

Indications d'application: Antigen Preadsorption Control: EC50 = ~2.2 pM

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. For storage of dilute working solutions, we recommend adding a carrier protein (e.g., 0.1 % BSA) to your buffer to prevent material loss due to protein adsorption. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Conseil sur la manipulation: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Stock: -20 °C

Stockage commentaire: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Détail du antigène

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Autre désignation: CNTF

Sujet: A potent survival factor for neurons and oligodendrocytes

Poids moléculaire: 22.7 kDa

UniProt: P20294

Pathways: Signalistation JAK/STAT