CD47 Protein (CD47) (AA 19-139)

N° du produit ABIN7504274

Protéine Recombinant CD47 exprimée dans HEK-293 Cells.

Aperçu rapide pour CD47 Protein (CD47) (AA 19-139) (ABIN7504274)

Antigène: CD47

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 19-139

Fonction: Human CD47 Protein

Séquence: Gln19-Pro139

Attributs du produit: Recombinant Human CD47 Protein is expressed from HEK293 without tag.It contains Gln19-Pro139.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD47, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.24ug/ml determined by ELISA.,Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD47

Autre désignation: CD47

Sujet: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Poids moléculaire: 13.72 kDa. Due to glycosylation, the protein migrates to 35-48 kDa based on Bis-Tris PAGE result.

UniProt: Q08722