ITCH Protein (AA 485-862)

N° du produit ABIN7504453

Cette protéine Recombinant ITCH est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour ITCH Protein (AA 485-862) (ABIN7504453)

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Détail du produit

Attributs du protein: AA 485-862

Fonction: Human ITCH Protein

Séquence: Arg485-Glu862

Attributs du produit: Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human ITCH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human ITCH is greater than 90% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Autre désignation: ITCH

Sujet: Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation.

Poids moléculaire: 44.98 kDa same as Bis-Tris PAGE result.

NCBI Accession: NP_113671

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events