CSF1R Protein (AA 20-512)

N° du produit ABIN7504465

Cette protéine Recombinant CSF1R est exprimée dans HEK-293 Cells.

Aperçu rapide pour CSF1R Protein (AA 20-512) (ABIN7504465)

Antigène: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 20-512

Fonction: Human M-CSF R/CSF1R/CD115 Protein

Séquence: Ile20-Glu512

Attributs du produit: Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 without tag.It contains Ile20-Glu512.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human M-CSF R, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).,Human M-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human M-CSF, His Tag on Anti-His-Biosensor can bind Human M-CSF R, No Tag with an affinity constant of 0.46 μM as determined in BLI assay (Gator® Prime).,The purity of Human M-CSF R is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Autre désignation: M-CSF R

Sujet: CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Poids moléculaire: 54.49 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.

UniProt: P07333

Pathways: Signalisation RTK, Inositol Metabolic Process, Cell-Cell Junction Organization