CD14 Protein (CD14) (His tag)
Aperçu rapide pour CD14 Protein (CD14) (His tag) (ABIN7534605)
Antigène
Voir toutes CD14 ProtéinesType de proteíne
Activité biologique
Origine
Source
Pureté
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Purification/Conjugué
- Cette CD14 protéine est marqué à la His tag.
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Fonction
- Active Recombinant Human CD14 Protein
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Séquence
- TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PAC
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Specificité
- Thr20-Cys352
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Stérilité
- 0.22 μm filtered
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niveau d'endotoxine
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 5.7-23 ng/mL.
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CD14 (CD14) (AA 16-345) protein (His tag)
CD14 Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
CD14 (CD14) (AA 20-171) protein (His-IF2DI Tag)CD14 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CD14 (CD14) (AA 20-352) protein (His tag)CD14 Origine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 18-345) (Active) protein (His-Fc tag)CD14 Origine: Souris Hôte: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
CD14 (CD14) (AA 20-352) (Active) protein (His tag)CD14 Origine: Humain Hôte: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
CD14 (CD14) (AA 20-344) protein (His tag)CD14 Origine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 20-349) protein (His tag)CD14 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
CD14 (CD14) protein (His tag,Fc Tag)CD14 Origine: Souris Hôte: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 Origine: Rat Hôte: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- CD14
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Autre désignation
- CD14
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Sujet
- CD14
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ID gène
- 929
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UniProt
- P08571
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Pathways
- Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Antigène
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