CIART (AA 1-375) protein (His tag)

N° du produit ABIN7563675

Protéine Recombinant CIART exprimée dans HEK-293 Cells.

Aperçu rapide pour CIART (AA 1-375) protein (His tag) (ABIN7563675)

Antigène: CIART

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Pureté: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Classe de qualité: custom-made

Détail du produit

Attributs du protein: AA 1-375

Purification/Conjugué: His tag

Fonction: Predefined custom protein recombinant Ciart Protein expressed in mammalian cells.

Séquence: MDSPSSVSSY SSSSLSPSFS TSSVNSDFSF PSDNEREGKG THELRPDTVG QRGGSRPSPG PIRCRHRPRV SSNQHTAPHL EQQGSEVKRS RDGEQETSLN TQGCTTEGDL LFAQKCKELQ GFIRPLTDLL NGLKMGRFDR GLSSFQQSVA MDRIQRIVGV LQKPQMGERY LGTLLQVEGM LKTWFPHIAA QKSSSGGSRH QISKHFPSHH GDPGAASPAP LLEKMGQTQL GHLVLKPKQP WHLTGWPAMN LTWIHSTPIC NPPLSSQGSA SGHSPIGTGA SIGVILVLQK GGQPFTHSAP GTPVPPTPLS PVVPGDLKKL PGEEPRCHSL PVTLPSDWSC ILCPPVLPTT DREMTKGHPE PQMTSHPPVA PDPQP Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificité: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Attributs du produit: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Information d'application

Indications d'application: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: 12 months

Détail du antigène

Antigène: CIART

Sujet: Circadian-associated transcriptional repressor (ChIP-derived repressor of network oscillator) (Chrono) (Computationally highlighted repressor of the network oscillator),FUNCTION: Transcriptional repressor which forms a negative regulatory component of the circadian clock and acts independently of the circadian transcriptional repressors: CRY1, CRY2 and BHLHE41. In a histone deacetylase-dependent manner represses the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Abrogates the interaction of BMAL1 with the transcriptional coactivator CREBBP and can repress the histone acetyl-transferase activity of the CLOCK-BMAL1 heterodimer, reducing histone acetylation of its target genes. Rhythmically binds the E-box elements (5'-CACGTG-3') on circadian gene promoters and its occupancy shows circadian oscillation antiphasic to BMAL1. Interacts with the glucocorticoid receptor (NR3C1) and contributes to the repressive function in the glucocorticoid response. {ECO:0000269|PubMed:24385426, ECO:0000269|PubMed:24736997, ECO:0000269|PubMed:24737000}.

Poids moléculaire: 40.4 kDa

UniProt: Q3TQ03

AlphaFold: Q3TQ03