NPHP1 Protein (AA 1-687) (His tag)

N° du produit ABIN7565155

Cette protéine Recombinant NPHP1 est produite dans HEK-293 Cells.

Aperçu rapide pour NPHP1 Protein (AA 1-687) (His tag) (ABIN7565155)

Antigène: NPHP1 (Nephronophthisis 1 (Juvenile) (NPHP1))

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Pureté: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Classe de qualité: custom-made

Détail du produit

Attributs du protein: AA 1-687

Purification/Conjugué: Cette NPHP1 protéine est marqué à la His tag.

Fonction: Predefined custom protein recombinant Nphp1 Protein expressed in mammalian cells.

Séquence: MLARRPRDPL QALRRRGQEL KLQVDSLVTE SQLTGALEPS KRREIYQRCI QLKQAVDENK NTLQKLNKAD EAAPVGNYEQ RKEEEHSLLE KLACQLQELA VSISRKDALK VEAHSDKEED DTTEDDEEET GGEEEESEGD GEGKQEQASP KQAETETVTY IALGDFAAQQ TGDLTFKKGD VLHIIEKKPD GWWLAKDAEG VEGLIPRTYL EPYNKEDKLE SSEGSEEGGE EDGEEDVEVV DETADGAQVK QRTDSHWSAV RKAISEQINT VDVLATMGAI PAGFRPSTLS QLLDEAGNQF RASYFLQPEL TTSQLAFRDL TWDAKAGTIM SRPSRVSLIL TLWSCKMIPL PGTSIQVLSR HIRLCLFDGS KVLSNIHTVR AVWQPKKPKT WTFSPQVTGI LPCLLDGDCF IRSNSSTPDL GILFELGISY IRNSTGERGE LSCGWVFLKL FDASGVPIPA KTYELFLNGG TPYEKGVEVD PSVSRRAQGS VFRQMISVRR QPQLLVKLRS LNRRSRAMLS LLPETLVGSM CSAHLLIFYR QILGDVLLRD RTNLQSADLI SHPVLATFPL LLEQPDVMDA LRSSWSEKES TLKRAEKRDK ELLKAEFLLV YHDCVLPLLH STLLPPFRWA EEETEAARWK AIADFLKQSR ENEGSLKALL SPDGVHKPFD LSEQTFDFLG EIRKNSG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificité: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Attributs du produit: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Information d'application

Indications d'application: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: 12 months

Détail du antigène

Antigène: NPHP1 (Nephronophthisis 1 (Juvenile) (NPHP1))

Autre désignation: Nphp1

Sujet: Nephrocystin-1,FUNCTION: Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells, together with NPHP4 and RPGRIP1L/NPHP8 (PubMed:21565611). Does not seem to be strictly required for ciliogenesis (PubMed:19208653). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling (PubMed:11493697). May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly (PubMed:19208653). Required for normal retina development (PubMed:19208653). In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19 (PubMed:19208653). Involved in spermatogenesis, required for the differentiation of early elongating spermatids into spermatozoa (PubMed:18684731). {ECO:0000269|PubMed:11493697, ECO:0000269|PubMed:18684731, ECO:0000269|PubMed:19208653, ECO:0000269|PubMed:21565611}.

Poids moléculaire: 77.0 kDa

UniProt: Q9QY53

AlphaFold: Q9QY53