IgE Protéine

N° du produit ABIN7596472

Cette protéine Recombinant IgE est produite dans Human.

Aperçu rapide pour IgE Protéine (ABIN7596472)

Antigène: IgE

Type de proteíne: Recombinant

Origine: Humain

Source: Human

Application: Western Blotting (WB), ELISA, Isotype Control (IsoC), Functional Studies (Func), Flow Cytometry (FACS)

Pureté: >90%, estimated from preparative Affinity Chromatography

Détail du produit

Clonalité: Chimeric

Fonction: Chimeric Human IgE (JW8)

Specificité: 4-Hydroxy-3-nitrophenylacetyl (NP)

Attributs du produit: Recombinant chimeric IgE consisting of a human Fc heavy chain linked to the antigen binding variable region of a mouse hybridoma by in vitro DNA recombination. The antibody is specific for the hapten 4-Hydroxy-3-iodo-5-nitrophenylacetyl (NIP). IgE (JW8) recognizes the human high affinity IgE receptor (FceRIa) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with NIP-BSA conjugate as specific cross-linking agent.

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – mouse anti-human IgE mAb (BSW17)

Stérilité: Sterile filtered

Information d'application

Indications d'application: ELISA, Flow Cytometry, functional cellular assays (mast cell / basophil activation).

Commentaires:

100% reactivity, compared to calibrator (Internal Standard IgE (JW8)), tested by SPR (Biacore™) binding to immobilized recombinant human high affinity IgE receptor

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) pH = 7.2

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4 °C. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thawing. Handle under sterile conditions, the mAb is formulated in sterile buffer solution and does not contain sodium azide as preservative. The product may be shipped on ice pack or dry ice.

Détail du antigène

Antigène: IgE