Adrenomedullin Protein (ADM) (His tag)

N° du produit ABIN7668126

Protéine Recombinant Adrenomedullin exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Adrenomedullin Protein (ADM) (His tag) (ABIN7668126)

Antigène: Adrenomedullin (ADM)

Type de proteíne: Recombinant

Activité biologique: Inactive

Origine: Rat

Source: Escherichia coli (E. coli)

Pureté: > 95 %

Détail du produit

Purification/Conjugué: Cette Adrenomedullin protéine est marqué à la His tag.

Fonction: Recombinant Rat Adrenomedullin/ADM protein (His tag)

Séquence: Thr 21-Leu 185

Purification: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 10 EU/mg of the protein as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Adrenomedullin (ADM)

Autre désignation: Adrenomedullin,ADM

Classe de substances: Hormone

Sujet: Adrenomedullin (ADM, also AM) is a secreted, monomeric, 6 kDa member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The ADM preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-91), the ADM precursor (amidation is required for maturation) (aa 94 143), and a C-terminal propeptide (aa 150-185). The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa 94 143).,ADM precursor,Adrenomedullin,PAMP,Preproadrenomedullin,ProAM N terminal 20 peptide,ProAM N20

Poids moléculaire:

calculated_mw: 18.0 kDa

observed_mw: 25 kDa

UniProt: P43145

Pathways: Hormone Transport, Hormone Activity, C21-Steroid Hormone Metabolic Process, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Tube Formation