Cathepsin B Protein (CTSB) (His tag)

N° du produit ABIN7668323

Cette protéine Recombinant Cathepsin B est exprimée dans Mammalian Cells.

Aperçu rapide pour Cathepsin B Protein (CTSB) (His tag) (ABIN7668323)

Antigène: Cathepsin B (CTSB)

Type de proteíne: Recombinant

Origine: Humain

Source: Mammalian Cells

Application: Immunogen (Imm)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Cathepsin B protéine est marqué à la His tag.

Fonction: Recombinant Human Cathepsin B Protein(His Tag)

Séquence: Met1-lle339

Purification: SDS-PAGE analysis of Human Cathepsin B proteins, 2μg/lane of Recombinant Human Cathepsin B proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 38-42 kDa

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Cathepsin B (CTSB)

Autre désignation: Cathepsin B

Sujet: APPS,CPSB,Cathepsin B,CTSB,APP Secretase,CathepsinB,CATB,Cathepsin B1,cysteine protease,Amyloid precursor protein secretase,Cathepsin B heavy chain,OTTHUMP00000116009,OTTHUMP00000229510,OTTHUMP00000229511,OTTHUMP00000229512,OTTHUMP00000229514,OTTHUMP00000229515,OTTHUMP00000229516,Preprocathepsin B,Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

Poids moléculaire:

Calculated MW: 37.2 kDa

Observed MW: 38-42 kDa

ID gène: 1508

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades