Midkine Protein (His tag)

N° du produit ABIN7669310

Cette protéine Recombinant Midkine est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Midkine Protein (His tag) (ABIN7669310)

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Type de proteíne: Recombinant

Activité biologique: Inactive

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 %

Détail du produit

Purification/Conjugué: Cette Midkine protéine est marqué à la His tag.

Fonction: Recombinant Human Midkine protein(N-His)

Séquence: Val 21-Asp 143

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.1 EU per μg of the protein as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Autre désignation: Midkine

Sujet: Midkine (MK or MDK) also known as neurite growth-promoting factor 2 (NEGF2) is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin. Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis. Midkine binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase and the induction of cell proliferation. Midkine is involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Midkine exhibited increased expression in the breast carcinomas but showed much lower expression in the normal breast tissue. Thus, it can be used as a breast carcinomas marker.,MK,NEGF-2

Poids moléculaire:

calculated_mw: 14.4 kDa

observed_mw: 18 kDa

UniProt: P21741

Pathways: Signalisation RTK, M Phase, Skeletal Muscle Fiber Development