14-3-3 sigma/SFN Protein (GST tag)

N° du produit ABIN7669789

Cette protéine Recombinant 14-3-3 sigma/SFN est produite dans Escherichia coli (E. coli).

Aperçu rapide pour 14-3-3 sigma/SFN Protein (GST tag) (ABIN7669789)

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Type de proteíne: Recombinant

Activité biologique: Inactive

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 94 %

Détail du produit

Purification/Conjugué: Cette 14-3-3 sigma/SFN protéine est marqué à la GST tag.

Fonction: Recombinant Human 14-3-3 sigma/YWHAS Protein (GST Tag)

Séquence: Met 1-Ser 248

Purification: > 94 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 10 mM GSH, 25 % glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Autre désignation: 14-3-3 sigma

Sujet: 14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.,14-3-3 Protein Sigma,Epithelial Cell Marker Protein 1,HME1,SFN,Stratifin

Poids moléculaire:

calculated_mw: 50.1 kDa

observed_mw: 48 kDa

UniProt: P31947

Pathways: Signalisation p53, Myometrial Relaxation and Contraction