CLDN18.2 protein (DYKDDDDK Tag)

N° du produit ABIN7853963

Cette protéine MNP Membrane Nanoparticle CLDN18.2 est produite dans HEK-293 Cells.

Aperçu rapide pour CLDN18.2 protein (DYKDDDDK Tag) (ABIN7853963)

Antigène: CLDN18.2

Type de proteíne: MNP Membrane Nanoparticle

Origine: Humain

Source: HEK-293 Cells

Détail du produit

Purification/Conjugué: DYKDDDDK Tag

Fonction: Human CLDN18.2 full length protein-MNP

Attributs du produit: Plasma membrane-coated nanoparticles (MNPs) have been used in various applications, including delivery of therapeutic agents and induction of immune responses et al. Unlike the conventional strategies, MNPs directly leverage intact and natural functions of cell membranes, and show high biocompatibility, specificity, and low side effects. Our optimized MNPs platform for the full-length membrane protein production uses membrane coating technology and a HEK293 based expression platform. The high-purity plasma membrane-coated nanoparticles were produced by extrusion after membrane extraction from the host HEK293 cells containing the overexpressed target proteins.

Information d'application

Commentaires:

Advantages of MNP:

• High display density of target membrane proteins
• Native structure and orientation of transmembrane protein
• soluble in aqueous solutions for routine biochemical analysis
• Detergent-free purification process
• Strong immunogenicity
• Works for MPs that can´t be produced via VLPs and EXos

Limitations of MNP:

• Lack of accurate quantification of the target membrane proteins.
• Need to develop special SPR assayx.
• Cell membranes contain housekeeping proteins that can result in immune response dilution.
• Some membrane proteins can´t be enriched on membrane.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from PBS. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: CLDN18.2

Sujet: SFTA5, SFTPJ
A member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]

Poids moléculaire: The human full length CLDN18.2 Protein has a MW of 27.5 kDa

UniProt: P56856-2