Sonic Hedgehog Protein (SHH) (N-Term)

N° du produit ABIN7956532

Cette protéine Recombinant Sonic Hedgehog est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Sonic Hedgehog Protein (SHH) (N-Term) (ABIN7956532)

Antigène: Sonic Hedgehog (SHH)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 98 % by SDS-PAGE and HPLC analyses.

Détail du produit

Attributs du protein: N-Term

Fonction: Recombinant Human Sonic Hedgehog N-Terminus

Séquence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 1 EU/ug of rHuSHH as determined by LAL method.

Biological Activity Comment: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 x10³ IU/mg.

Information d'application

Commentaires:

Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Date de péremption: 12 months

Détail du antigène

Antigène: Sonic Hedgehog (SHH)

Autre désignation: Sonic Hedgehog

Poids moléculaire: 19.8 kDa

ID gène: 6469

UniProt: Q15465

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development