NOG Protéine

N° du produit ABIN7937428

Protéine Recombinant NOG exprimée dans Cellules d'insectes.

Aperçu rapide pour NOG Protéine (ABIN7937428)

Antigène: NOG (Noggin (NOG))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Cellules d'insectes

Pureté: > 95 % by SDS-PAGE and HPLC analyses.

Détail du produit

Fonction: Recombinant Human Noggin, Insect Cells Derived

Séquence: QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 EU/ug of rHuNoggin, Insect Cell as determined by LAL method.

Biological Activity Comment: Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.

Information d'application

Commentaires:

Approximately 23.0 kDa on SDS-PAGE under reducing conditions, containing 205 amino acids, and a molecular mass about 47.9 kDa homodimer under non-reducing conditions (Molecular size on SDS-PAGE will appear at approximately 50-80 kDa).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5 % trehalose, 0.02 %Tween-20.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Date de péremption: 12 months

Détail du antigène

Antigène: NOG (Noggin (NOG))

Autre désignation: Noggin

Poids moléculaire: 23.0 kDa,47.9 kDa,80 kDa

ID gène: 9241

UniProt: Q13253

Pathways: Stem Cell Maintenance, Tube Formation