IL-2 Protein (C145S)

N° du produit ABIN934929

Cette protéine Recombinant IL-2 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-2 Protein (C145S) (ABIN934929)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 % pure

Détail du produit

Attributs du protein: C145S

Séquence: MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT

Attributs du produit: Purified recombinant Human IL2 protein
Expression System: E.coli
Bioactivity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/mL.
Molecular weight on SDS-PAGE will appear higher.

Information d'application

Indications d'application: IL2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Procédure de l'essai:

Cell line: CTLL2 (mouse cytotoxic T-cell) Maintenance Condition: 10 % FBS RPMI 1640 with hIL2 Assay medium: 10 % FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Starvation: 24 h, Assay medium Incubation Time: 24 h (after sample treatment) Concentration Range: 0.039 ng/mL - 40 ng/mL Detection method: MTT assay

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Stockage commentaire: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Détail du antigène

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL2

Sujet: IL-2, also known as T cell growth factor(TCGF), is an immunoregulatory lymphokine produced by T-cells in response to protein or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: T-cell growth factor protein, Interleukin 2 protein, IL-2, IL-2 protein, IL 2 protein, IL-2 protein, Aldesleukin protein, Interleukin-2 protein, IL 2, IL2, IL 2 protein, TCGF protein

Poids moléculaire: 15.4 kDa (133 AA)

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation