CCL18 Protéine

N° du produit ABIN988166

Cette protéine Recombinant CCL18 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour CCL18 Protéine (ABIN988166)

Antigène: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 97 % by SDS-PAGE and HPLC analyses.

Détail du produit

Séquence: AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLN

Attributs du produit: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.

niveau d'endotoxine: Level Less than 1EU/µg of rHuMIP-4/CCL18 as determined by LAL method

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stock: 4 °C

Détail du antigène

Antigène: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Autre désignation: MIP-4/CCL18

Sujet: CCL18, is a novel CC chemokine that is highly homologous to MIP-1ª (61% amino acid sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-γ. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils. Synonym: MIP-4/CCL18, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.

Poids moléculaire: 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.